Diagnostically significant single nucleotide polymorphisms in the <I>env</I>-gene locus for SNP-genotyping of <I>Bovine leukemia virus</I>

Relevance . The etiological agent in the development of persistent lymphocytosis and lymphosarcoma cattle is Bovine leukemia virus Phylogenetic analysis sequenced nucleotide sequences BLV env -gene locus main, but not only, approach to pathogen genotypic classification. Metods aim study was identify single-nucleotide polymorphisms -gene, considered as diagnostically significant for SNP genotyping based on direct sequencing data PCR-product. Results Alignment 101 type isolates known genotypes revealed 155 single polymorphisms, which 35 were significant. At same time, SNP-genotyping, an 22 characterized sufficient, 14 are genotype-specific relation nine studied viral (C232G 3rd, G84A 4th, A117G, T326C C412T — 5th, C394T 8th, C40G 9th, C132T 10th, T113A T256C 11th, T49C G140A 12th, C83A T304C 13th genotypes, thereby ensuring their identification. other 8 polymorphic positions genotype-specific, interpretation results detection has identification value remaining 4 genotypes: 1st (G205G A337A), 2nd (T220C A334G), 6th (A127R, C132C, T153C C341C) 7th A334A) respectively.